The Effect of Standardized Mangosteen Skin Extraction, Nano-emulsion, Nano-chitosan and Treadmill Exercise on Atherogenic Rat Model
DOI:
https://doi.org/10.36408/mhjcm.v11i3.1182Keywords:
Mangosteen skin, nano-emulsion, nano-chitosan, treadmill exercise, atherogenesisAbstract
Background: Atherosclerosis, the most common cause of CVD, is associated with oxidative stress and cholesterol. Antioxidant and regular physical exercise have been considered as the probable interventions to dampen the process. The pericarp of mangosteen (Garcinia mangostana Linn) is known for containing a high amount of xanthones including α-mangostin with antioxidant effects. The current study investigates the anti-atherogenic potential of mangosteen skin extract in nano-emulsion and nano-chitosan formulations, singly and in combination with treadmill exercise, versus the statin, Atorvastatin.
Methods: A randomized controlled trial was conducted on 30 male Wistar rats, divided into six groups: normal diet control (C1), atherogenic diet control (C2), and four treatment groups receiving an atherogenic diet plus treadmill exercise combined with Atorvastatin (T1), standardized mangosteen extract (T2), mangosteen nano-emulsion (T3), or mangosteen nano-chitosan (T4). The aortic tunica intima and tunica intima-media thickness were measured histologically after 56 days.
Results: The aortic intimal thickness was noticeably higher in the atherogenic diet group (C2: 12.13±1.87 mm) compared to the normal diet group (C1: 4.27±0.75 mm). In the treatment groups, the intimal thickness ranged from 2.97±0.45 mm to 4.17±1.70 mm and showed no significant differences from the normal diet group. A similar pattern was seen in the intima-media thickness, with 145.63±17.12 mm recorded for the normal diet group and values ranging from 106.90±10.41 mm to 135.90±12.63 mm in the treatment groups, again without significant differences. Survival analysis using Kaplan-Meier curves showed clear differences between the groups (p < 0.001). The untreated atherogenic diet group (C2) had the poorest survival, with no rats surviving until the study's conclusion. In contrast, survival improved in all treatment groups, with the Mangosteen Nano-chitosan (T4) group and the normal diet group (C1) achieving the best outcomes, as all rats in these groups survived.
Conclusion: Mangosteen skin extracts, whether in nano-emulsion or nano-chitosan forms, combined with treadmill exercise, showed significant differences in maintaining the survivability of rat with atherogenic-induced diet despite no significant differences in preventing atherogenesis compared to Atorvastatin or a normal diet. Further research is needed to confirm these potential therapeutic effects.
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References
1. Herrmann J, Lerman LO, Mukhopadhyay D, Napoli C, Lerman A. Angiogenesis in atherogenesis. Arterioscler Thromb Vasc Biol. 2006 Sep 1;26(9):1948–57.
2. Falk E. Pathogenesis of atherosclerosis. J Am Coll Cardiol. 2006 Apr 18;47(8 Suppl).
3. Zhao Y, Qu H, Wang Y, Xiao W, Zhang Y, Shi D. Small rodent models of atherosclerosis. Biomed Pharmacother. 2020 Sep 1;129.
4. Bauersachs R, Zeymer U, Brière JB, Marre C, Bowrin K, Huelsebeck M. Burden of Coronary Artery Disease and Peripheral Artery Disease: A Literature Review. Cardiovasc Ther. 2019;2019.
5. Libby P. Changing concepts of atherogenesis. J Intern Med. 2000 Mar 1;247(3):349–58.
6. Severino P, D’amato A, Pucci M, Infusino F, Adamo F, Birtolo LI, et al. Ischemic Heart Disease Pathophysiology Paradigms Overview: From Plaque Activation to Microvascular Dysfunction. Int J Mol Sci. 2020 Nov 1;21(21):1–30.
7. Schwenke, D. C. (1998). Antioxidants and atherogenesis. https://doi.org/https://doi.org/10.1016/S0955-2863(98)00046-1
8. Peñín-Grandes S, Martín-Hernández J, Valenzuela PL, López-Ortiz S, Pinto-Fraga J, Solá L del R, et al. Exercise and the hallmarks of peripheral arterial disease. Atherosclerosis. 2022 Jun 1;350:41–50.
9. Boonprom P, Boonla O, Chayaburakul K, Welbat JU, Pannangpetch P, Kukongviriyapan U, et al. Garcinia mangostana pericarp extract protects against oxidative stress and cardiovascular remodeling via suppression of p47phox and iNOS in nitric oxide deficient rats. Ann Anat. 2017 Jul 1;212:27–36.
10. Sari N, Katanasaka Y, Sugiyama Y, Miyazaki Y, Sunagawa Y, Funamoto M, et al. Alpha Mangostin Derived from Garcinia magostana Linn Ameliorates Cardiomyocyte Hypertrophy and Fibroblast Phenotypes in Vitro. Biol Pharm Bull. 2021 Oct 1;44(10):1465–72.
11. Wihastuti TA, Aini FN, Tjahjono CT, Heriansyah T. Dietary Ethanolic Extract of Mangosteen pericarp Reduces VCAM-1, Perivascular Adipose Tissue and Aortic Intimal Medial Thickness in Hypercholesterolemic Rat Model. Open Access Maced J Med Sci. 2019 Oct 15;7(19):3158–63.
12. Alam M, Rashid S, Fatima K, Adnan M, Shafie A, Akhtar MS, et al. Biochemical features and therapeutic potential of α-Mangostin: Mechanism of action, medicinal values, and health benefits. Biomedicine & Pharmacotherapy. 2023 Jul 1;163:114710.
13. Setiawan AA, Budiman J, Prasetyo A. Anti-Inflammatory Potency of Mangosteen (Garcinia mangostana L.): A Systematic Review. Open Access Maced J Med Sci. 2023 Jan 2 [cited 2024 Aug 29];11(F):58–66.
14. Kumar V, Bhatt PC, Kaithwas G, Rashid M, Al-abbasi FA, Khan JAJ, et al. α-Mangostin Mediated Pharmacological Modulation of Hepatic Carbohydrate Metabolism in Diabetes Induced Wistar Rat. Beni Suef Univ J Basic Appl Sci. 2016 Sep;5(3):255–76.
15. Wihastuti TA, Sargowo D, Heriansyah T, Aziza YE, Puspitarini D, Iwana AN, et al. The reduction of aorta histopathological images through inhibition of reactive oxygen species formation in hypercholesterolemia rattus norvegicus treated with polysaccharide peptide of Ganoderma lucidum. Iran J Basic Med Sci. 2015;18(5):514.
16. Wathoni N, Rusdin A, Motoyama K, Joni IM, Lesmana R, Muchtaridi M. Nanoparticle Drug Delivery Systems for α-Mangostin. Nanotechnol Sci Appl. 2020;13:23.
17. Chacon D, Fiani B. A Review of Mechanisms on the Beneficial Effect of Exercise on Atherosclerosis. Cureus. 2020 Nov 23;12(11).
18. Bowles DK, Laughlin MH. Mechanism of Beneficial Effects of Physical Activity on Atherosclerosis and Coronary Heart Disease: Mechanism of beneficial effects of physical activity on atherosclerosis and coronary heart disease. J Appl Physiol. 2011 Jul;111(1):308.
19. Frasier CR, Moore RL, Brown DA. Exercise-induced cardiac preconditioning: how exercise protects your achy-breaky heart. J Appl Physiol (1985). 2011 Sep [cited 2024 Aug 29];111(3):905–15.
20. Niebauer J, Cooke JP. Cardiovascular effects of exercise: role of endothelial shear stress. J Am Coll Cardiol. 1996;28(7):1652–60.
21. Mika P, Konik A, Januszek R, Petriczek T, Mika A, Nowobilski R, et al. Comparison of two treadmill training programs on walking ability and endothelial function in intermittent claudication. Int J Cardiol. 2013 Sep 30;168(2):838–42.
22. Grundy SM, Stone NJ, Bailey AL, Beam C, Birtcher KK, Blumenthal RS, et al. 2018 AHA/ACC/AACVPR/AAPA/ABC/ACPM/ADA/AGS/APhA/ASPC/NLA/PCNA Guideline on the Management of Blood Cholesterol: A Report of the American College of Cardiology/American Heart Association Task Force on Clinical Practice Guidelines. Circulation. 2019 Jun 18;139(25):E1082–143.
23. Wihastuti TA, Widodo MA, Heriansyah T, Sari NAK. Study of the inhibition effect of ethanolic extract of mangosteen pericarp on atherogenesis in hypercholesterolemic rat. Asian Pac J Trop Dis. 2015 Oct 1;5(10):830–4.
24. Feriani A, Bizzarri M, Tir M, Aldawood N, Alobaid H, Allagui MS, et al. High-fat diet-induced aggravation of cardiovascular impairment in permethrin-treated Wistar rats. Ecotoxicol Environ Saf. 2021 Oct 1;222:112461.
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