In Vitro Testing of the Antibacterial Activity of Ethanol Extract of Lontar Leaves (Borassus flabellifer) Against Staphylococcus aureus

Authors

  • Ami Febriza Achmad Department of Physiology, Faculty Medicine and Health Sciences, Universitas Muhammadiyah Makassar, Department of Surgery, Faculty Medicine and Health Sciences, Universitas Muhammadiyah Makassar, Indonesia https://orcid.org/0000-0002-0283-1727
  • Asdar Tajuddin Department of Surgery, Faculty Medicine and Health Sciences, Universitas Muhammadiyah Makassar, Indonesia
  • Rifqah Amaliah Faculty Medicine and Health Sciences, Universitas Muhammadiyah Makassar, Indonesia
  • Rolly Riksanto Bahtiar Faculty Medicine and Health Sciences, Universitas Muhammadiyah Makassar, Indonesia https://orcid.org/0009-0005-8879-5454

DOI:

https://doi.org/10.36408/mhjcm.v12i3.1228

Keywords:

Borassus flabellifer, antibacterial activity, Staphylococcus aureus, herbal extract, in vitro study

Abstract

BACKGROUND: Staphylococcus aureus is a bacterium that frequently causes infections and often develops resistance to antibiotics. Efforts to identify alternative treatments using herbal remedies are increasing. In Indonesia, a country rich in biodiversity, lontar leaves (Borassus flabellifer) have been traditionally used and show potential antibacterial activity.

AIMS: To evaluate the antibacterial activity of ethanol extract of lontar leaves against Staphylococcus aureus in vitro.

METHOD: This experimental study employed a post-test-only control group design. Antibacterial activity was tested using the well diffusion method. Extracts were obtained through maceration with 96% ethanol and tested at 75%, 50%, and 25% concentrations. Ciprofloxacin was used as the positive control, and 10% DMSO as the negative control.

RESULTS: The inhibition zone diameters were 21.86 mm (75%), 19.64 mm (50%), and 18.37 mm (25%). The positive control (ciprofloxacin) measured 24.43 mm, while the negative control (DMSO) showed 0 mm.

CONCLUSION: The 96% ethanol extract of lontar leaves demonstrated antibacterial activity against Staphylococcus aureus, with higher concentrations yielding stronger inhibition.

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Additional Files

Published

2025-11-28

How to Cite

1.
Achmad AF, Tajuddin A, Amaliah R, Bahtiar RR. In Vitro Testing of the Antibacterial Activity of Ethanol Extract of Lontar Leaves (Borassus flabellifer) Against Staphylococcus aureus. Medica Hospitalia J. Clin. Med. [Internet]. 2025 Nov. 28 [cited 2025 Dec. 4];12(3):315-20. Available from: https://medicahospitalia.rskariadi.co.id/index.php/mh/article/view/1228

Issue

Vol. 12 No. 3 (2025): Med Hosp; Nov 2025

Section

Original Article

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Copyright (c) 2025 Ami Febriza Achmad, Asdar Tajuddin, Rifqah Amaliah, Rolly Riksanto Bahtiar (Author)

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