The Increased Superoxide Dismuatase (SOD) In Mice Infected by Plasmodium Berghei ANKA Treated with Nanoparticle Extract of Beetroot (Beta Vulgaris L)
DOI:
https://doi.org/10.36408/mhjcm.v11i2.1119Keywords:
Superoxide dismutase, malaria, beetroot, nanoparticle, antioxidantAbstract
BACKGROUND: Malaria infection causes increased free radicals which leads to severity and decreases antioxidant activity, thus increasing the risk of severe malaria complications. Beetroot extract has active compounds that function as anti-inflammatory and antioxidants. Nanoparticles are a technology that can be used to improve drug delivery efficiency in smaller doses.
AIMS: to prove the effectiveness of beetroot extract nanoparticles on SOD levels in mice infected with malaria and treated with artemisinin
METHOD: An experimental study using a post-test-only randomized control group design. The research sample used 30 male Balb/c mice divided into 6 groups. Group 1 was the healthy group, group 2 was the infected group without treatment, group 3 was the infected group with artemisinin treatment, group 4 was the infected group with artemisinin treatment and 50 mg/kgBW/day beetroot extract nanoparticles, group 5 was the infected group with artemisinin treatment and 100 mg/kg BW/day beetroot extract nanoparticles, and group 6 was the infected group with artemisinin treatment and 200 mg/kg BW/day beetroot extract nanoparticles. Beetroot extract and artemisinin supplementation were given after parasitemia index > 1% and given for 4 days. On the 5th day after therapy, serum SOD levels were measured using ELISA.
RESULT: The measurement of SOD levels in the artemisinin group supplemented with nanoparticle extracts of beetroot at doses 100-200 mg/KgBW were 21,48-21,59 ng/ml. Kruskal Wallis and Mann Whitney test showed that they are significantly higher serum SOD levels compared to the infected mice group (p<0.05).
CONCLUSION: Supplementation of beetroot extract nanoparticles has an antioxidant effect by increasing SOD levels in mice infected with malaria and receiving artemisinin therapy.
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References
1. World Malaria Report 2021. Word Malaria Report Geneva: World Health Organization. 2021
2. Vasquez M, Zuniga M & Rodriguez A. Oxidative Stress and Pathogenesis in Malaria. Cell. Infect. Microbiol.2021;11:1–8. Retrieved (https://doi.org/10.3389/fcimb.2021.768182)
3. Percário S, Moreira DR, Gomes B, et al. Oxidative stress in Malaria. J. Mol. Sci. 2012: 13; 16346–72. Retrieved (https://doi.org/10.3390/ijms131216346)
4. Andrade B. Reis-Filho A, Souza-Neto SM, et al. Plasma Superoxide Dismutase-1 as A Surrogate Marker of Vivax Malaria Severity. PLoS Negl. Trop. Dis. 2010;4(4): e650. Retrieved (https://doi.org/10.1371/journal.pntd.0000650)
5. Liliana C, Oana-Viorela N. Red Beetroot: Composition And Health Effects - A Review. Nutr. Med. Diet Care. 2020:6(1);1-95. Retrieved (https://doi.org/10.23937/2572-3278.1510043)
6. Clifford T, Howatson G, West DJ, & Stevenson EJ. The Potential Benefits Of Red Beetroot Supplementation In Health And Disease. Nutrients. 2015; 7: 2801-22. Retrieved (https://doi.org/10.3390/nu7042801)
7. Rubi DS, Pramana ACC, Sunarti. The Protective Effects of Red Beetroot (Beta vulgaris L) Againts Oxidative Stress in Rats Induced by High Fat and Fructose Diet. Acta Biochim. Indones. 2020:3; 62–70. Retrieved (https://doi.org/10.32889/actabioina.v3i2.53)
8. Thakur SR, Agrawal R. Application of Nanotechnology In Pharmaceutical Formulation Design And )
9. Tiwari G, et al. Drug Delivery Systems: An Updated Review. J. Pharm. Investig. 2012;2: 2. Retrieved (http://dx.doi.org/10.4103/2230-973X.96920)
10. Ghorani B, Naji-Tabasi S, Bostan A, Emadzadeh B. Application Of Nanotechnology In The Safe Delivery Of Bioactive Compounds.US: CRC Press Taylor and Francis Group. 2019;12: 237-92
11. Hardimarta FP, Ikawati K, Yuniarti CA. The improved appearance of atherosclerotic lesions by administering beta vulgaris extract to mice on an atherogenic diet model. Media Farm. Indones. 2020;15(1):1571–7. Retrieved (https://doi.org/10.53359/mfi.v15i1.140
12. Albasher G, et al. Nephroprotective Role Of Beta Vulgaris L. Root Extract Against Chlorpyrifos-Induced Renal Injury In Rats. Evidence-Based Complement. Med. 2019. Article ID 3595761;1-912. Retrieved (https://doi.org/10.1155/2019/3595761)
13. Al Ezzi A. A, Al Salahy M, Shnawa B, et al. Changes in Levels of Antioxidant Markers and Status of Some Enzyme Activities among Falciparum Malaria Patients in Yemen. Microbiol. Exp.2017: 4, 4–7. Retrieved (https://doi.org/10.15406/jmen.2017.04.00131)
14. Raza A,Varshney S.K, Khan H.M,et al. Superoxide Dismutase Activity In Patients Of Cerebral Malaria. Asian Pacific J. Trop. Dis. 2015; 5: S51-3 15. Retrieved (https://doi.org/10.1016/S2222-1808(15)60856-8)
15. Kavishe RA, Koenderink JB, Alifrangis M. Oxidative Stress in Malaria and Artemisinin Combination Therapy: Pros and Cons. FEBS J. 2017:284; 2579-91. Retrieved (https://doi.org/10.1111/febs.14097)
16. Sadowska-Bartosz I, Bartosz G. Biological Properties and Applications of Betalains. Molecules. 2021: 26; 1–36. Retrieved (https://doi.org/10.3390%2Fmolecules26092520)
17. Nahla T K, Wisam S U, Tariq NM. Antioxidant Activities of Beetroot (Beta vulgaris L.) Extracts. Pakistan J. Nutr. 2018:17; 500–5. Retrieved (https://doi.org/10.3923/pjn.2018.500.505)
18. Ngo V, Duennwald ML. Nrf2 and Oxidative Stress: A General Overview of Mechanisms and Implications in Human Disease. Antioxidants. 2022:11:2345. Retrieved (https://doi.org/10.3390/antiox11122345)
19. Chen L, Zhu Y, Hu Z, Wu S, Jin C. Beetroot as A Functional F ood with Huge Health Benefits: Antioxidant, Antitumor, Physical Function, and Chronic Metabolomics Activity. Food Sci. Nutr. 2021: 9; 6406–20. Retrieved (https://doi.org/10.1002%2Ffsn3.2577)
20. Milton-Laskibar I, Alfredo Martínez J, Portillo MP. Current Knowledge on Beetroot Bioactive Compounds: Role of Nitrate and Betalains in Health and Disease. Foods. 2021: 10;1–14. Retrieved (https://doi.org/10.3390%2Ffoods10061314)
21. da Silva DVT, Baião D, Ferreira VF, Paschoalin VMF. Betanin as A Multipath Oxidative Stress and Inflammation Modulator: A Beetroot Pigment with Protective Effects on Cardiovascular Disease Pathogenesis. Rev. Food Sci. Nutr. 2021: 62; 539-54. Retrieved (https://doi.org/10.1080/10408398.2020.1822277)
22. Bucur L, Taralunga G, Schroder V. The betalains content and antioxidant capacity of red beet (Beta vulgaris L. subsp. vulgaris) root. Farmacia. 2016: 64: 198–201
23. Czapski J, Mikolajczyk K, Kaczmarek M. Relationship between antioxidant capacity of red beet juice and contents of its betalain pigments. Polish J. Food Nutr. Sci. 2009: 59:119-22
24. Albohiri HH, Al-Zanbagi NA, Alzahrani MS, Albohairi SH, Alsulami MN, Abdel-Gaber R, et al. Evaluation of antiplasmodial potential of Beta vulgaris juice in Plasmodium berghei infected mice. J. King Saud Univ. - Sci. 2022: 34: 101844. https://doi.org/10.1016/j.jksus.2022.101844
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