Perbedaan Jumlah Trombosit, Leukosit Dan Eritrosit Dengan Kecepatan Sentrifugasi Yang Berbeda Pada Pembuatan Platelet Rich Plasma

Authors

  • Dwi Fajaryani Patologi Klinik Fakultas Kedokteran Universitas Diponegoro, Indonesia
  • Muji Rahayu Bagian Patologi Klinis RSUP Dr. Kariadi Semarang, Indonesia
  • Edward Kurnia Setiawan Limijadi Bagian Patologi Klinik Fakultas Kedokteran Universitas Diponegoro/RSUP Dr. Kariadi, Indonesia

DOI:

https://doi.org/10.36408/mhjcm.v7i1.421

Keywords:

PRP, trombosit, leukosit, eritosit

Abstract

Latar belakang : Platelet Rich Plasma (PRP) adalah plasma dalam jumlah sedikit dengan jumlah trombosit banyak, didapatkan melalui sentrifugasi. Trombosit  dalam PRP penting untuk penyembuhan luka dan memperbaiki jaringan rusak. Kecepatan sentrifugasi berperan penting dalam memisahkan sel darah merah dengan plasma dan menghasilkan platelet konsentrat. Penelitian ini bertujuan untuk mengetahui perbedaan jumlah trombosit, leukosit dan eritrosit dengan kecepatan sentrifugasi yang  berbeda pada pembuatan platelet rich plasma.

Metode: Penelitian belah lintang pada orang sehat di laboratorium RSUP Dr. Kariadi Semarang  bulan Juli 2018. Darah vena ditampung dalam  tabung sitras  dan Ethylenediaminetetraacetic acid (EDTA). Darah EDTA untuk pemeriksaan hematologi rutin dengan hematology analyzer, hasil normal dilanjutkan pembuatan PRP dari darah sitras 3,2 %  dengan dua metode sentrifugasi, dengan kecepatan 100xg-400x selama 10 menit (metode PRP1) dan kecepatan 540xg-2270xg selama 10 menit (metode PRP2). Kedua produk PRP dilakukan pemeriksaan  jumlah trombosit, leukosit dan eritrosit. Data dianalisis dengan uji Kruskall Wallis dan Mann Whitney, signifikan bila p< 0.05.

Hasil : Sampel terdiri dari 35 orang responden. Terdapat perbedaan bermakna jumlah trombosit, leukosit dan eritrosit pada pembuatan PRP-1 dan PRP-2  (p<0.001)

Simpulan : Terdapat perbedaan jumlah trombosit, leukosit dan eritrosit pada kecepatan sentrifugasi yang berbeda. Kecepatan sentrifugasi pada metode pembuatan PRP disarankan dalam pembuatan PRP.

Kata kunci : PRP, trombosit, leukosit, eritrosit

 

Background : Platelet Rich Plasma (PRP) consisting of small amounts plasma with many platelet, which was obtained by centrifugation process. The platelet in PRP are essential for wound healing and repair of damaged tissue. The centrifugation velocity plays an important role in separating red blood cells by plasma and producing concentrated-platelets. This study aims to determine differences the number of platelets, leukocytes and erythrocytes with different centrifugation rates on platelet rich plasma production.

Methods: This cross sectional study was conducted on 35 healthy people in the laboratory of Kariadi Hospital Semarang in July 2018.  The blood vein were collected  in Citrate 3.2 % and Ethylenediaminetetraacetic acid (EDTA) tubes. Sample EDTA for complete blood counts, if the results were normal then continued with PRP preparation in 2 different methods centrifugation (PRP 1 at 100xg-400xg for 10 minutes and PRP 2 at 540xg-2270 xg for 10 minutes). Both of the PRP products were calculated the amounts of platelet, leukocytes, and erythrocytes. The data were analyzed by Kruskall Wallis and Mann Whitney, p < 0.05 was considered as significant.

Results : There was a significant difference between PRP-1 and PRP-2 method in platelet counts, leukocytes count and erythrocytes count (p<0.001).

Conclusion : There was differences in platelet count, leukocytes count and erythrocytes count with different centrifugation rate. Centrigugation rate on PRP-1 method is recommended in the production of PRP.

Keyword

PRP, platelets, leukocytes, erythrocytes

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References

1. Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85:638–46.
2. Lee JW, Kwon OH, Kim TK, Cho YK, Choi KY, Chung HY, et al. Platelet-Rich Plasma: Quantitative Assessment of Growth Factor Levels and Comparative Analysis of Activated and Inactivated Groups. Arch Plast Surg. 2013;40:530-5.
3. Khalafi RS, Bradford DW, Wilson MG. Topical application of autologous blood product during surgical closure, folowing a coronary artery bypass graft. Eur J Cardiothorac Surg. 2008;34:360-4.
4. Budiyanto A. Penggunaan PRP di bidang dermatologi. Bagian/SMF Ilmu kesehatan Kulit dan kelamin. FK UGM/RSUP Dr. Sardjito Yogyakarta. Available from : http://andinanendra.wordpress.com/2011/prp-for-skin-rejuvent
5. Perez AGM, Lana JFSD, Rodrigues AA, Luzo ACM, Belangero WD, Santana MHA. Relevant aspects of centrifugation step in the preparation of platelet rich plasma. Hindawi. ISRN Hematology. 2014.
6. Kamruzzaman BM, Hasan A , Dhingra N. Standard Operating Procedures for blood tranfusion, Directorate general of health services (BANBCT), Mokhali Technical Assistance by WHO and Supported by The OPEC Fondation for International Development, 2013. Available from www.who.int/bloodsafety /tranfusion_service/sop-bts_bangladesh

7. Plao L, Park H, Jo CH. Theoritical prediction and validation of cell recovery rates in preparing platelet rich plasma through a centrifugation. PLOS ONE. 2017;12(11): 1-25.
8. Kaushansky K, Lichtman MA, Kipps TJ, Prchal JT, Levi MM. Williams Hematology eight edition, 2010.
9. Bausset O, Giraudo L, Veran J, Magalon J, Coudreuse JM, Magalon G, et al. Formulation and storage of platelet rich plasma homemade product. BioResearch Open Access, Mary Ann Liebert, Inc. 2012;1(3):115-23.
10. Nugraha HK, Muljanti M, Hernaningsih Y, Nugraha J. Platelet rich plasma protocols : A Preliminary Study. Indonesia journal of tropical and infectious disease. 2012;3(2):104-7
11. Raturi M, Shastry S, Raj P. Cumulative quality asessment for whole Blood-derived platelets : a complience review. Global Journal of Transfusion Medicine. 2017;2:38-43.
12. Satyam A. Preparation and quality control of autologous platelets for
therapeutic applications. Clinical Association Departement of Tranfusion
Medicine. BLK Super Speciality Hospital, NewDelhi. 2015. Available from :https://www.researchgate.net

Additional Files

Published

2020-05-15

How to Cite

1.
Fajaryani D, Rahayu M, Limijadi EKS. Perbedaan Jumlah Trombosit, Leukosit Dan Eritrosit Dengan Kecepatan Sentrifugasi Yang Berbeda Pada Pembuatan Platelet Rich Plasma. Medica Hospitalia J. Clin. Med. [Internet]. 2020 May 15 [cited 2024 Dec. 24];7(1):12-6. Available from: http://medicahospitalia.rskariadi.co.id/medicahospitalia/index.php/mh/article/view/421

Issue

Vol. 7 No. 1 (2020): Med Hosp; Mei 2020

Section

Original Article

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