AUTOLOGOUS FULL THICKNESS SKIN GRAFT WOUND HEALING ON SPRAGUE DAWLEY RATS Reviewed From TGF-? Expression And Neutrophil Number
DOI:
https://doi.org/10.36408/mhjcm.v8i3.489Keywords:
Ozonated VCO, Autologous Full Thickness Skin Graft, TGF-? expression, number of neutrophilsAbstract
Abstract
Introduction : Skin grafts are now one of treatment option in wound healing process that is always developing. TGF-bexpression and the number of neutrophils have an important role in healing skin graft wounds. Ozone (O3) has disinfecting properties that are effective in wound healing.
Objective : Proving the effectiveness of Ozonated VCO for Full Thickness Skin Graft wound healing using parameter of TGF-b expression and neutrophil number.
Method : This study is an experimental study with a post-test only design group of 40 Sprague Dawley rats performed autologous skin graft at the same time. Samples were divided randomly into 8 groups (K1 = without Ozonated VCO), (A1 = Ozonated VCO 50 mg / ml), (B1 = Ozonated VCO 100 mg / ml), (C1 = Ozonated VCO 200 mg / ml), ( K2 = without Ozonated VCO) (A2 = Ozonated VCO 50 mg / ml), (B2 = Ozonated VCO 100 mg / ml), (C2 = Ozonated VCO 200 mg / ml). Assessment of TGF-b expression and neutrophil number of tissue was performed by staining hematoxylin & eosin and immunohistochemistry on days 6 and 12 after skin graft.
Results : There were significant differences (p <0.05) TGF-b expression and neutrophils number of tissue between the control group and the administration of Ozonated VCO doses of 50 mg / ml, 100 mg / ml and 200 mg / ml on days 6 and 12 post skin graft.
Conclusion : The administration of Ozonated VCO effectively improve Full Thickness Skin Graft wound healing seen from macroscopic wounds, increase TGF-b expression and decrease the number of neutrophils.
Downloads
References
REFERENSI
Robson MC, Steed DL, Franz MG. Wound healing: Biologic features and approaches to maximize healing trajectories. Curr Probl Surg. 2001 Available from: http://www.ncbi.nlm.nih.gov/pubmed/11452260
Schreml S, Szeimies RM, Prantl L, Karrer S, Landthaler M, Babilas P. Oxygen in acute and chronic wound healing. Br J Dermatol. 2010. Available from: http://www.ncbi.nlm.nih.gov/pubmed/20394633
Dhivya S, Padma VV, Santhini E. Wound dressings - a review. BioMedicine. 2015. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26615539
HTA Indonesia. Terapi Ozon. 2004.
Zhang J, Guan M, Xie C, Luo X, Zhang Q, Xue Y. Increased growth factors play a role in wound healing promoted by non invasive oxygen-ozone therapy in diabetic patients with foot ulcers. Oxid Med Cell Longev. 2014.
Valacchi G, Lim Y, Belmonte G, et. al. Ozonated sesame oil enhances cutaneous wound healing in SKH1 mice. Wound Repair and Regeneration. 2010.
Velnar T, Bailey T, Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. J Int Med Res. 2009.
Wyatt J. Forensic Medicine. New York: Oxford University Press; 2011.
Co?kun S, Güleç EG, Balabanli B, Acartürk F. Effects of epidermal growth factor on lipid peroxidation and nitric oxide levels in oral mucosal ulcer healing: a time-course study. Surg Today. 2007. Available from: http://www.ncbi.nlm.nih.gov/pubmed/17593476
Diegelmann RF. Wound healing: an overview of acute, fibrotic and delayed healing. Front Biosci. 2004.
Penn JW, Grobbelaar AO, Rolfe KJ. The role of the TGF-? family in wound healing, burns and scarring: a review. Int J Burn Trauma. 2012. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3415964
Guo S, DiPietro LA. Factors Affecting Wound Healing. J Dent Res. 2010.
Gonzalez AC de O, Costa TF, Andrade Z de A, Medrado ARAP. Wound healing - A literature review. An Bras Dermatol. 2016. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27828635
Kawasumi A, Sagawa N, Hayashi S, Yokoyama H, Tamura K. Wound healing in mammals and amphibians: toward limb regeneration in mammals. Curr Top Microbiol Immunol. 2012. 367:33–49.
Orsted Heather L. et all KDH. Skin: Anatomy, Physiology and Wound Healing. canada: Canadian Association of Wound Care ; 2018. p. 7–23.
De Monte A, Gori C. Major ozonated autohemotherapy in the treatment of limb ulcers not responding to conventional therapy. Vol. 10, International Journal of Ozone Therapy. 2011.
Degli AI, Ginelli E, Mazzacane B, et al. Effectiveness of a Short-Term Treatment of Oxygen-Ozone Therapy into Healing in a Posttraumatic Wound. Case Rep Med. 2016.
Soelistijo SA, Novida H, Rudijanto A, Soewondo P, Suastika K, Manaf A, et al. Konsensus Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 Di Indonesia. PB Perkeni. Jakarta: PB Perkeni; 2015.
American Diabetes Association. Classification and Diagnosis of Diabetes. In: Diabetes Care. 2016. p. 513–22.
Powers AC. Diabetes Mellitus: Introduction. In: Fauci A, Braunwald E, Isselbacher K, Wilson J, Martin J, Kasper D, editors. Harrison’s Principles of Internal Medicine. 17th ed. New York: McGraw Hill; 2008.
Wilgus TA, Roy S, McDaniel JC. Neutrophils and Wound Repair: Positive Actions and Negative Reactions. Adv Wound Care (New Rochelle). 2014.
Qing C. The molecular biology in wound healing & non-healing wound. Chinese J Traumatology. 2017. Available from: http://www.ncbi.nlm.nih.gov/pubmed/28712679
Shaw TJ, Martin P. Wound repair at a glance. J Cell Sci. 2009/09/04. 2009;122(Pt 18):3209–13.
Gurtner GC, Werner S, Barrandon Y, Longaker MT. Wound repair and regeneration. Nature [Internet]. 2008 May 15 [cited 2019 Jan 10];453(7193):314–21. Available from: http://www.nature.com/doifinder/10.1038/nature07039
Borges GÁ, Elias ST, da Silva SMM, et al. In vitro evaluation of wound healing and antimicrobial potential of ozone therapy. J Cranio-Maxillofacial Surg [Internet]. 2017;45(3):364–70. Available from: http://dx.doi.org/10.1016/j.jcms.2017.01.005
Shah JMY, Omar E, Pai DR. Cellular events and biomarkers of wound healing. Indian J Plast Surg [Internet]. 2012 May [cited 2018 Dec 17];45(2):220–8. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23162220
Antoniades HN, Scher CD, Stiles CD. Purification of human platelet-derived growth factor. Proc Natl Acad Sci USA. Available from: http://www.ncbi.nlm.nih.gov/pubmed/287022
Bocci VA. Scientific and medical aspects of ozone therapy. State of the art. Arch Med Res. 2006 May;37(4):425–35.
Elvis AM, Ekta JS. Ozone therapy: A clinical review. J Nat Sci Biol Med. 2011 Jan;2(1):66–70.
Zanardi I, Borrelli E, Valacchi G, Travagli V, Bocci V. Ozone: A Multifaceted Molecule with Unexpected Therapeutic Activity. Curr Med Chem. 2016;23(4):304–14.
Additional Files
Published
How to Cite
Issue
Section
Citation Check
License
Copyright (c) 2021 Medica Hospitalia : Journal of Clinical Medicine
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Copyrights Notice
Copyrights:
Researchers publishing manuscrips at Medica Hospitalis: Journal of Clinical Medicine agree with regulations as follow:
Copyrights of each article belong to researchers, and it is likewise the patent rights
Researchers admit that Medica Hospitalia: Journal of Clinical Medicine has the right of first publication
Researchers may submit manuscripts separately, manage non exclusive distribution of published manuscripts into other versions (such as: being sent to researchers’ institutional repository, publication in the books, etc), admitting that manuscripts have been firstly published at Medica Hospitalia: Journal of Clinical Medicine
License:
Medica Hospitalia: Journal of Clinical Medicine is disseminated based on provisions of Creative Common Attribution-Share Alike 4.0 Internasional It allows individuals to duplicate and disseminate manuscripts in any formats, to alter, compose and make derivatives of manuscripts for any purpose. You are not allowed to use manuscripts for commercial purposes. You should properly acknowledge, reference links, and state that alterations have been made. You can do so in proper ways, but it does not hint that the licensors support you or your usage.
