AUTOLOGOUS FULL THICKNESS SKIN GRAFT WOUND HEALING ON SPRAGUE DAWLEY RATS Reviewed From TGF-? Expression And Neutrophil Number

Authors

  • Nauval Marta Kusuma Magister Biomedik dan Bagian Bedah Fakultas Kedokteran Universitas Diponegoro, Semarang, Indonesia
  • Hardian Hardian Bagian Faal Fakultas Kedokteran Universitas Diponegoro, Semarang, Indonesia
  • Najatullah Najatullah Bagian Bedah Plastik Fakultas Kedokteran Universitas Diponegoro, Semarang, Indonesia
  • Renni Yuniati Bagian Kulit dan Kelamin Fakultas Kedokteran Universitas Diponegoro, Semarang, Indonesia
  • Neni Susilaningsih Bagian Histologi Fakultas Kedokteran Universitas Diponegoro, Semarang, Indonesia

DOI:

https://doi.org/10.36408/mhjcm.v8i3.489

Keywords:

Ozonated VCO, Autologous Full Thickness Skin Graft, TGF-? expression, number of neutrophils

Abstract

Abstract

Introduction : Skin grafts are now one of treatment option in wound healing process that is always developing. TGF-bexpression and the number of neutrophils have an important role in healing skin graft wounds. Ozone (O3) has disinfecting properties that are effective in wound healing.

Objective : Proving the effectiveness of Ozonated VCO for Full Thickness Skin Graft wound healing using parameter of TGF-b expression and neutrophil number.

Method : This study is an experimental study with a post-test only design group of 40 Sprague Dawley rats performed autologous skin graft at the same time. Samples were divided randomly into 8 groups (K1 = without Ozonated VCO), (A1 = Ozonated VCO 50 mg / ml), (B1 = Ozonated VCO 100 mg / ml), (C1 = Ozonated VCO 200 mg / ml), ( K2 = without Ozonated VCO) (A2 = Ozonated VCO 50 mg / ml), (B2 = Ozonated VCO 100 mg / ml), (C2 = Ozonated VCO 200 mg / ml). Assessment of TGF-b expression and neutrophil number of tissue was performed by staining hematoxylin & eosin and immunohistochemistry on days 6 and 12 after skin graft.

Results : There were significant differences (p <0.05) TGF-b expression and neutrophils number of tissue between the control group and the administration of Ozonated VCO doses of 50 mg / ml, 100 mg / ml and 200 mg / ml on days 6 and 12 post skin graft.

Conclusion : The administration of Ozonated VCO effectively improve Full Thickness Skin Graft wound healing seen from macroscopic wounds, increase TGF-b expression and decrease the number of neutrophils.

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References

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Additional Files

Published

2021-11-05

How to Cite

1.
Kusuma NM, Hardian H, Najatullah N, Yuniati R, Susilaningsih N. AUTOLOGOUS FULL THICKNESS SKIN GRAFT WOUND HEALING ON SPRAGUE DAWLEY RATS Reviewed From TGF-? Expression And Neutrophil Number. Medica Hospitalia J. Clin. Med. [Internet]. 2021 Nov. 5 [cited 2024 Mar. 28];8(3):317-25. Available from: http://medicahospitalia.rskariadi.co.id/medicahospitalia/index.php/mh/article/view/489

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