The Association Between Variants of Angiotensin Converting Enzyme (ACE) Gene With Risk Factors in Patients with Ischemic Stroke at Dr. Kariadi Semarang

Authors

  • Dodik Tugasworo Pramukarso Departemen Neurologi Fakultas Kedokteran Universitas Diponegoro / RSUP Dr. Kariadi, Indonesia
  • Herlina Suryawati Departemen Neurologi Fakultas Kedokteran Universitas Diponegoro / RSUP Dr. Kariadi, Indonesia
  • Soetedjo Soetedjo Departemen Neurologi Fakultas Kedokteran Universitas Diponegoro / RSUP Dr. Kariadi, Indonesia
  • Jimmy Eko Budi Hartono Departemen Neurologi Fakultas Kedokteran Universitas Diponegoro / RSUP Dr. Kariadi, Indonesia
  • Trianggoro Budisulistyo Departemen Neurologi Fakultas Kedokteran Universitas Diponegoro / RSUP Dr. Kariadi, Indonesia
  • Arinta Puspita Wati Departemen Neurologi Fakultas Kedokteran Universitas Diponegoro / RSUP Dr. Kariadi, Indonesia
  • Aditya Kurnianto Departemen Neurologi Fakultas Kedokteran Universitas Diponegoro / RSUP Dr. Kariadi, Indonesia
  • Patria Adri Wibhawa Departemen Neurologi Fakultas Kedokteran Universitas Diponegoro / RSUP Dr. Kariadi, Indonesia

DOI:

https://doi.org/10.36408/mhjcm.v8i3.565

Keywords:

ACE; Angiotensin Converting Enzyme; ischemic stroke

Abstract

BACKGROUND : Stroke is the leading cause of death and disability in the world. The occurrence of ischemic stroke is indicated by genetic factors, environmental factors and the interaction between it. Angiotensin Converting Enzyme (ACE) genetic variant is associated with various characteristics of risk factors for ischemic stroke.

OBJECTIVE : Identifying genetic variants of Angiotensin Converting Enzyme (ACE) with the Polymerase Chain Reaction (PCR) method and to find it’s correlation beetwen risk factor in patients with Ischemic Stroke at Dr. Kariadi Semarang

METHOD : The subjects of the study were 72 patients with ishcemic stroke who were treated at the polyclinic of the Neurology Department Dr. Kariadi Semarang in January - December 2013. DNA extraction of research subjects was carried out at the Laboratory of the Center of Biomedical Research (CEBIOR), Faculty of Medicine, Diponegoro University from January to March 2020. Amplification using the Polymerase Chain Reaction (PCR) method was carried out using an Eppendorf thermocycler. Data were analyzed with SPSS for Windows Version 25

RESULT : 72 samples analyzed obtained genetic variants of ACE II 39 (54.2%) samples, genetic variants of ACE DI 30 samples (41.7%), and genetic variants of ACE DD 3 (4.2%) samples. Meanwhile, there was no significant relationship ( p>0,05 ) between genetic variants of ACE and the characteristics of risk factors for ischemic stroke, namely age, gender, Body Mass Index, smoking history, triglyceride levels, HDL levels, LDL levels, obesity and hypertension.

CONCLUSION : There are three types of ACE genetic variants, including the ACE II genetic variant, the ACE DI genetic variant, and the ACE DD genetic variant. Among the three genetic variants, ACE II genetic variant is the most common variant and there is no significant relationship to the various risk factor characteristics found in ischemic stroke patients at Dr. Kariadi Gneeral Hospital Semarang.

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References

Zhong L-L, Ding L-S, He W, Tian X-Y, Cao H, Song Y-Q, et al. Systolic hypertension related single nucleotide polymorphism is associated with susceptibility of ischemic stroke. European Review for Medical and Pharmacological Sciences. 2017; 21: 2901-6

Rasyid A, Hidayat R, Harris S, Kurniawan M, Mesiano T. Stroke iskemik. In: Aninditha T, Wiratman W, editors. Buku ajar neurologi. Tangerang: Penerbit Kedokteran Indonesia; 2017. p. 452-75.

Wang X, Cheng S, Brophy VH, Erlich HA, Mannhalter C, Berger K, et al. A meta-analysis of candidate gene polymorphism and ischemic stroke in six study populations: association of lymphotoxin-alpha in non-hypertensive patients. Stroke. 2009 March; 40(3): 683-95

Morishita R, Gibbons GH, Ellison KE, Lee W, Zhang L, et al. (1994) Evidence for direct local effect of angiotensin in vascular hypertrophy. In vivo gene transfer of angiotensin converting enzyme. J Clin Invest 94: 978–984.

Pitt B (1994) Angiotensin-converting enzyme inhibitors in patients with coronary atherosclerosis. Am Heart J 128: 1328–1332.

Raynolds MV, Bristow MR, Bush EW, Abraham WT, Lowes BD, et al. (1993) Angiotensin-converting enzyme DD genotype in patients with ischaemic or idiopathic dilated cardiomyopathy. Lancet 342: 1073–1075.

B Rigat, C Hubert, F Alhenc-Gelas, F Cambien, P Corvol, et al. An insertion/deletion polymorphism in the angiotensin I-converting enzyme gene accounting for half the variance of serum enzyme levels. J Clin Invest. ( 1990 ) Oct;86(4):1343-6

Yulan Zhao, Chunlan Xu. Structure And Function Of Angiotensin Converting Enyme And its Inhibitors. Chinese Journal Of Biotech. 2008; 24(2):171-176

Islam MS Lehtimaki T Juonala M Kahonen M Hutri-Kahonen N Kainulainen K et al. Polymorphism of the angiotensin-converting enzyme (ACE) and angiotesinogen (AGT) genes and their associations with blood pressure and carotid artery intima media thickness among healthy Finnish young adults ? The Cardiovascular risk in young Finns study. Atherosclerosis. 2006; 188(2): 316-322

17. Kogawa K Nishizawa Y Hosoi M Kawagishi T Maekawa K Shoji T et al. Effect of polymorphism of apolipoprotein E and angiotensin-converting enzyme genes on arterial wall thickness. Diabetes 1997; 46(4): 682-687

Purnamasari D Widjojo BD Antono D Syampurnawati M. ACE gene polymorphism and atherosclerotic lesion of carotid artery among offsprings of type 2 diabetes mellitus. Acta Med Indones. 2012; 44(2): 128-134

Hong SH, Park HM, Ahn JY, Kim OJ, Hwang TS et al. ACE I/D Polymrphysm In Korean Patients With Ishemic Stroke And Silent Brain Infarction. Acta Neurol Scand. 2008 : 117 : 244 – 249

Douglas M. Bennion, Christian A Rosad, Emily A Haltigan, Robert W Regenhardt, Colin Sumners, Michael F. Walters. Serum Activuty Of Angioensin Converting Enzyme 2 Is Decreased In Patients With Acute Ischemic Stroke. Journal Of Renin – Angiotensin Aldosteron System. 2016 ; 1-7

Bennion DM, Haltigan EA, Irwin AJ, et al. Activation Of The Neuroprotective Angiotensin – Converting Enzyme 2 In Rat Ischemic Stroke. Hypertension 2015; 66 : 141 – 148

31. PENA – Silva RA, Heistad DD. Stages In Discovery : Angiotensin – Converting Enzyme Type 2 And Stroke. Hypertension 2015; 66 : 15 – 16

32. Jiang F, Yag J, Zhang Y at al. Angiotensin – Converting Enzyme 2 And Angiotensin 1 – 7 : Novel Therapeutic Targets. Nat Rev Cardiol 2014;11 : 413-426

Additional Files

Published

2021-11-05

How to Cite

1.
Pramukarso DT, Suryawati H, Soetedjo S, Hartono JEB, Budisulistyo T, Wati AP, Kurnianto A, Wibhawa PA. The Association Between Variants of Angiotensin Converting Enzyme (ACE) Gene With Risk Factors in Patients with Ischemic Stroke at Dr. Kariadi Semarang. Medica Hospitalia J. Clin. Med. [Internet]. 2021 Nov. 5 [cited 2024 Apr. 19];8(3):297-303. Available from: http://medicahospitalia.rskariadi.co.id/medicahospitalia/index.php/mh/article/view/565

Issue

Vol. 8 No. 3 (2021): Med Hosp; Nov 2021

Section

Original Article

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