Analysis of Genetic Variation of Angiotensinogen M235T Gene in Ischemic Stroke Patients treated at Dr. Kariadi General Hospital, Semarang using Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) Method

Authors

  • Dodik Tugasworo Department of Neurology, Dr. Kariadi Hospital/Faculty of Medicine Diponegoro University, Semarang, Indonesia, Indonesia
  • Retnaningsih Retnaningsih Department of Neurology, Dr. Kariadi Hospital/Faculty of Medicine Diponegoro University, Semarang, Indonesia, Indonesia
  • Aditya Kurnianto Bagian Neurologi Fakultas Kedokteran Universitas Diponegoro/RSUP dr Kariadi Semarang, Indonesia
  • Suryadi Suryadi Department of Neurology, Dr. Kariadi Hospital/Faculty of Medicine Diponegoro University, Semarang, Indonesia, Indonesia
  • Dani Rahmawati Department of Neurology, Dr. Kariadi Hospital/Faculty of Medicine Diponegoro University, Semarang, Indonesia, Indonesia
  • Rahmayanti Rahmayanti Department of Neurology, Dr. Kariadi Hospital/Faculty of Medicine Diponegoro University, Semarang, Indonesia, Indonesia
  • Jethro Budiman Department of Neurology, Dr. Kariadi Hospital/Faculty of Medicine Diponegoro University, Semarang, Indonesia, Indonesia

DOI:

https://doi.org/10.36408/mhjcm.v10i1.833

Keywords:

AGT M235T, Angiotensinogen, hypertension, ischemic stroke

Abstract

Background: Stroke is the leading cause of death and disability in the world. The incidence of ischemic stroke is influenced by genetic factors, environmental factors and their interactions. Genetic variation of the Angiotensinogen (AGT) M235T gene is associated with hypertension and diabetes mellitus, which are risk factors for stroke.

Objective: To examine the genetic variation of the Angiotensinogen M235T gene in patients with Ischemic Stroke treated at Dr. Kariadi General hospital, Semarang.

Method: The subjects of the study were 72 ischemic stroke patients who were treated at the outpatient clinic of the Neurology Department Dr. Kariadi Semarang in January - December 2013. DNA extraction of research subjects was performed at the CEBIOR laboratory, Diponegoro National Hospital from January to March 2020. Amplification was performed with Polymerase Chain Reaction (PCR). Digestion of PCR products was using Restriction Fragment Length Polymorphism (RFLP) method.

Results: Out of 72 samples, the AGT M235T CT were found in 37 samples (51.4%), the AGT M235T TT gene was found in 35 samples (48.6%) and no samples showed the AGT M235T CC.

Conclusion: There are 3 types of genetic variants of the AGT M235T gene, including the AGT M235T CT, the AGT M235T TT and the AGT M235T CC. Among the three types of variants, the variant of the AGT M235T CT gene is the most common variant found in ischemic stroke patients treated at the Dr. Kariadi General Hospital Semarang.

Downloads

Download data is not yet available.

References

  1. Zhong L-L, Ding L-S, He W, Tian X-Y, Cao H, Song Y-Q, et al. Systolic hypertension related single nucleotide polymorphism is associated with susceptibility of ischemic stroke. European Review for Medical and Pharmacological Sciences. 2017; 21: 2901-6.
  2. Rasyid A, Hidayat R, Harris S, Kurniawan M, Mesiano T. Stroke iskemik. In: Aninditha T, Wiratman W, editors. Buku ajar neurologi. Tangerang: Penerbit Kedokteran Indonesia; 2017. p. 452-75.
  3. Wang X, Cheng S, Brophy VH, Erlich HA, Mannhalter C, Berger K, et al. A meta-analysis of candidate gene polymorphism and ischemic stroke in six study populations: association of lymphotoxin-alpha in non-hypertensive patients. Stroke. 2009 March; 40(3): 683-95.
  4. Al-Najai M, Muiya P, Tahir AI, Elhawari S, Gueco D, Andres E, et al. Association of the angiotensinogen gene polymorphism with atherosclerosis and its risk traits in the Saudi population. BMC Cardiovasc Disord. 2013;13(1):1.
  5. Padma G, Charita B, Swapna N, Mamata M, Padma T. Novel variants detected in AGT gene among patients with essential hypertension. 2015;1–5.
  6. Shaikh R, Shahid SM, Mansoor Q, Ismail M, Azhar A. Genetic variants of ACE ( Insertion / Deletion ) and AGT ( M268T ) genes in patients with diabetes and nephropathy. 2014.
  7. Lu H, Cassis LA, Kooi CW Vander, Daugherty A. Structure and functions of angiotensinogen. 2016;39(7):492–500.
  8. Dickson ME, Sigmund CD. Genetic basis of hypertension: Revisiting angiotensinogen. Hypertension. 2006;48(1):14–20.
  9. Corvol P, Inserm U. Molecular Genetics of Human Hypertension : Role of. 1997;18(5):662–77.
  10. PubChem [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; 2004-. PubChem Gene Summary for Gene 183, AGT - angiotensinogen (human); [cited 2022 Oct. 29]. Available from: https://pubchem.ncbi.nlm.nih.gov/gene/AGT/human
  11. Procopciuc L, Popescu T, Jebeleanu G, Pop D, Zdrenghea D. Essential arterial hypertension and polymorphism of angiotensinogen M235T gene. J Cell Mol Med. 2002;6(2):245–50.
  12. Ehret GB, Caulfield MJ. Genes for blood pressure: An opportunity to understand hypertension. Eur Heart J. 2013;34(13):951–61.
  13. Yamasan BE, Gulyasar T, Sipahi T, Sivri N, Palabiyik O. Investigation of Angiotensinogen M235T and T174M Gene Polymorphisms in Coronary Artery Disease. Erciyes Med J. 2021;43(5):475–81.
  14. Elalfy MS, Soliman F, Ebeid E, Kamal TM, Eissa DS, Abdel E, et al. Angiotensinogen M235T Gene Polymorphism is a Genetic Determinant of Cerebrovascular and Cardiopulmonary Morbidity in Adolescents with Sickle Cell Disease. J Stroke Cerebrovasc Dis. 2019;28(2):441–9.
  15. Mondry A, Loh M, Liu P, Zhu A-L, Nagel M. Polymorphisms of the insertion / deletion ACE and M235T AGT genes and hypertension : surprising new findings and meta-analysis of data. 2005;11:1–11.
  16. Raharjo S, Chahyadi A, Fadhilah A, Kholidha AN. Hubungan polimorfisme gen angiotensinogen m235t dengan hipertensi esensial pada etnis sulawesi tenggara. Semin Nas Ris Kuantitatif Terap 2017. 2017;(April):16–20.
  17. Shamaa MM, Fouad H, Haroun M, Hassanein M, Ayman M, Hay A. Association between the Angiotensinogen (AGT) gene (M235T) polymorphism and Essential Hypertension in Egyptian patients. Egypt Heart J. 2015;67(1):1-5.
  18. Gao T, Huang L, Fu Q, Bai Y. Association of polymorphisms in the AGT gene(M235T, T174M) with ischemic stroke in the Chinese population. JRAAS - J Renin-Angiotensin-Aldosterone Syst. 2015;16(3):681–6.
  19. Kooffreh ME, Kumar PL. A study of the M235T variant of the angiotensinogen gene and hypertension in a sample population of Calabar and Uyo, Nigeria. Egypt J Med Hum Genet. 2013;14(1):13–9.

Additional Files

Published

2023-03-31

How to Cite

1.
Tugasworo D, Retnaningsih R, Kurnianto A, Suryadi S, Rahmawati D, Rahmayanti R, Budiman J. Analysis of Genetic Variation of Angiotensinogen M235T Gene in Ischemic Stroke Patients treated at Dr. Kariadi General Hospital, Semarang using Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) Method. Medica Hospitalia J. Clin. Med. [Internet]. 2023 Mar. 31 [cited 2024 May 3];10(1):63-8. Available from: http://medicahospitalia.rskariadi.co.id/medicahospitalia/index.php/mh/article/view/833

Issue

Vol. 10 No. 1 (2023): Med Hosp; March 2023

Section

Original Article

Citation Check

License

Copyright (c) 2023 Dodik Tugasworo, Retnaningsih, Aris Catur Bintoro, Suryadi, Aditya Kurnianto

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyrights Notice
Copyrights:
Researchers publishing manuscrips at Medica Hospitalis: Journal of Clinical Medicine agree with regulations as follow:
Copyrights of each article belong to researchers, and it is likewise the patent rights
Researchers admit that Medica Hospitalia: Journal of Clinical Medicine has the right of first publication
Researchers may submit manuscripts separately, manage non exclusive distribution of published manuscripts into other versions (such as: being sent to researchers’ institutional repository, publication in the books, etc), admitting that manuscripts have been firstly published at Medica Hospitalia: Journal of Clinical Medicine
License:
Medica Hospitalia: Journal of Clinical Medicine is disseminated based on provisions of Creative Common Attribution-Share Alike 4.0 Internasional It allows individuals to duplicate and disseminate manuscripts in any formats, to alter, compose and make derivatives of manuscripts for any purpose. You are not allowed to use manuscripts for commercial purposes. You should properly acknowledge, reference links, and state that alterations have been made. You can do so in proper ways, but it does not hint that the licensors support you or your usage.