The Effectivity of Ozonated VCO on Healing Full Grafted Skin graft Study on Progenitor Cell Endothelial Proliferation and Angiogenesis in Sprague Dawley Mice

Authors

  • Rollin Hansen Sidauruk Magister Ilmu Biomedik Fakultas Kedokteran Universitas Diponegoro, Indonesia
  • Renni Yuniati Bagian Bedah Onkologi Ilmu Biomedik Fakultas Kedokteran Universitas Diponegoro/ RSUP Dr. Kariadi, Indonesia
  • Djoko Handojo Bagian Bedah Onkologi Ilmu Biomedik Fakultas Kedokteran Universitas Diponegoro/ RSUP Dr. Kariadi, Indonesia

DOI:

https://doi.org/10.36408/mhjcm.v8i2.446

Keywords:

Full thickness skin graft, Ozonated oil, EPC, Angiogenesis

Abstract

Abstract

 

Background : Full thickness  Skin graft is one technique to accelerate wound healing. EPC proliferation and new blood vessel formation have an important role in the success of Ful thickness  Skin graft. Ozone content in Ozonated VCO  has the ability to increase growth factors so that it can accelerate wound healing   Aims : This study was aimed to prove the effects of ozonated vco  in various doses in increasing EPC proliferation and angiogenesis in full thickness skin graft wounds.   Methods : This research is an experimental study with randomized post test only with parallel group design. 40 Sprague Dawley rats performed autologous  Skin grafts at the same time. Samples were randomly divided into 8 groups, two control groups (X1 and X5), and 6 other groups were given a multi-dose ozonated oil  50.6 mg / ml (X2, X6), 101.3 mg / ml (X3, X7) , 202.6 mg / ml (X4, X8). Conduct termination on day 6 and day 12. Examination of the number of  EPC proliferation and the number of angiogenesis in each group. Data were analyzed by Saphiro Wijk, ANOVA, post hoc benferonni and pearsons   Results : The number of EPC proliferation in groups X1, X2, X3, X4, X5, X6, X7, X8 has a mean of 6.2 + 0.837, 10 + 1.581, 13.80 + 2.049, 14.80+ 2,280, 9.40 + 2,074 , 13.20 + 1,483, 19,0 + 2,055 and 19.20 + 1,095.The number of Angiogenesis in groups X5, X6, X7, X8 has a mean of 9.20 + 1,095,14,60 + 2,881,17,40+ 3,286, 18,80 + 2,168,17,40 + 3,286,18.80+ 2,168. The administration of ozonated VCO  dose of 202.6 mg / ml gave the best results in the amount of angiogenesis (p = 0.001) and the amount of angiogenesis (p=0,001).Correlation analysis between EPC proliferation and angiogenesis showed a significant correlation (p = 0.001 and r = 0.738).   Conclusion : Ozonated oil can increase the number of EPC proliferation and angiogenesis in the wound healng process of Full thickness  Skin graft. The ozone dose of 202,6 mg / ml showed the best effect on increase wound healing process. Keyword : Full thickness skin graft, Ozonated oil , EPC, Angiogenesis

 

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References

Velnar T, Bailey T, Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. J Int Med Res. 2009;37(5):1528-42

Geoffrey C, Victor W. Wound Healing: Normal and Abnormal. In: Thorne CH, editor. Grabb and Smith's Plastic Surgery. 7 ed. Philadelphia: Lippincott Williams & Wilkins. 2014; 20-35.

Harper D, Young A, McNaught C-E. The physiology of wound healing. Basic Science. Surgery 2014;32(9):445–50.

Lee CK, Hansen SL. Management of acute wound. Surg Clin North Am. 2009; 89(3):659-76.

Thorne CH. Technique and Principles in Plastic Suqery. In: Thorne CH, editor. Grabb and Smith's Plastic Surgery. 7 ed. Philadelphia: Lippincott William . Wilkins. 2014; 1-19.

I. C. Valencia, A. F. Falabella, W. H. Eaglstein. Skin grafting. Dermatologic Clinics 2000;18(3.):521-32.

Kim, H.S., Noh, S.U., Han, Y.W., Kim, K.M., Kang, H., Kim, H. O, et al. Therapeutic Effects Of Topical Application Of Ozon On Acute Cutaneous Wound Healing. J Korean Med Sci. 2009; 24: 368-74.

Aggarwal B, Lamba HS, Sharma P, Ajeer. Various Pharacological Aspects of Cocos nucifera-A Review. J Pharmacol Sci. 2017: 5:25-30.

Rajagukguk H, Syukur S, Ibrahim S, Syafrizayanti S. Beneficial Effect of Application of Virgin Coconut oil (VCO) Product from Padang West Sumatra, Indonesia on Palatoplasty Wound Healing. ASRJETS. 2017: 34:231-6.

Ghani NAA, Channip A-A, Hwa PCH, Ja’Faar F, Yasin HM, Usman A. Physicochemical properties, antioxidant capacities, and metal content of virgin coconut oil produced by wet and dry processes. Food Sci Nutr J. 2018: 6:1298-306.

Smith NL, Wilson AL, Gandhi J, Vatsia S, Khan SA. Ozon Therapy: an overview of pharmacodynamics, current research, and clinical utility. Med Gas Res J. 2017; 7(3): 212–219.

Valacchi G, Zanardi I, Lim Y, Belmonte G, Miracco C, Sticozzi C, et al. Ozonated sesame oil enhances cutaneous wound healing in SKH1 mice. Int J Pharm. 2013; 458(1):65-73.

George, A. L., Bangalore-Prakash, P., Rajoria, S., Suriano, R., Shanmugam, A., Mittelman, A. et al. Endothelial Progenitor Cell Biology In Disease And Tissue Regeneration. J Hematol Oncol 2011;4(1):24-31.

Napoli C, Paolisso G, Casamassimi A, Al-Omran M, Barbieri M, Sommese L, et al. MD, et all. Effects of nitric oxide on cell proliferation: novel insights. J Am Coll Cardiol 2013;62(2):89-95.

Tepper O, Capla J, Galiano R, Ceradini D, Callaghan M, Kleinman M, et al. Adult vasculogenesis occurs through in situ recruitment, proliferation, and tubulization of circulating bone marrow-derived cells. Blood. 2005;105(3);1068-7.

Li W, Liu ZQ, Wei J, Liu Y, Hu LS. Contribution of endothelial progenitor cells to neovascularization (Review). International J of Molecular Medicine 2012;30(5):1000-6.

Yang Y, Qi PK, Yang ZL, Huang N. Nitric oxide based strategies for applications of biomedical devices. Biosurface and Biotribology1 2015;177–201.

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Published

2021-07-15

How to Cite

1.
Sidauruk RH, Yuniati R, Handojo D. The Effectivity of Ozonated VCO on Healing Full Grafted Skin graft Study on Progenitor Cell Endothelial Proliferation and Angiogenesis in Sprague Dawley Mice. Medica Hospitalia J. Clin. Med. [Internet]. 2021 Jul. 15 [cited 2024 Mar. 29];8(2):133-8. Available from: http://medicahospitalia.rskariadi.co.id/medicahospitalia/index.php/mh/article/view/446

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