EFFECTIVENESS OF OZONATED VIRGIN COCONUT OIL ON FULL THICKNESS SKIN DEFECT WOUND HEALING IN SPRAGUE DAWLEY Rats

  • Herry Maha Putra Surbakti Magister Ilmu Biomedik – Konsentrasi Klinik Bedah Fakultas Kedokteran Universitas Diponegoro
  • Renni Yuniati Magister Ilmu Biomedik Fakultas Kedokteran Universitas Diponegoro
  • Djoko Handojo Bedah Onkologi Ilmu Biomedik Fakultas Kedokteran Universitas Diponegoro/ RSUP Dr. Kariadi, Semarang
Keywords: Virgin Coconut Oil, full thickness defect, TGF-beta, Wound size

Abstract

Abstract
Introduction: Wounds are a breakdown of tissue integrity / continuity that can lead to infection. Virgin Coconut Oil is a processed coconut product made by processing fresh coconut flesh at low temperature and has a high nutritional content. Ozone therapy is an alternative therapy that has disinfectant properties and can induce strong oxidative stress.

Methods: This study was conducted on 50 Sprague-Dawley rats that had injuries. We performed full thickness wounds and administered ozonated doses of VCO to mice. We looked at shrinkage of wounds and TGF Beta levels in mice. Assessment was carried out on day 7 and day 14 to assess the wound and TGF-beta immunohistochemically.

Results: There was a significant difference in the wound shrinkage variable between the negative control group and the group that received VCO offerings both on day 7 and day 14. We also found a significant difference in the TGF-beta variable between the negative control group and the group that received VCO offerings both on day 7 and day 14.

Conclusion: The administration of ozonated virgin coconut oil was effective in increasing the expression of TGF-? in the full thickness defect of Sprague Dawley rats and was effective in increasing the reduction in wound size in the full thickness defect of Sprague Dawley rats.

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References

Dhivya S, Padma VV, Santhini E. Wound dressings - a review. BioMedicine. 2015 Dec;5(4):22.

Geoffrey C, Victor W. Wound Healing: Normal and Abnormal. In: Thorne CH, editor. Grabb and Smith’s Plastic Surgery. 7th ed. Philadelphia: Lippincott Williams & Wilkins; 2014.

Pastar I, Stojadinovic O, Yin NC, Ramirez H, Nusbaum AG, Sawaya A, et al. Epithelialization in Wound Healing: A Comprehensive Review. Adv Wound Care. 2014;3(7).

Gurtner GC, Werner S, Barrandon Y, Longaker MT. Wound repair and regeneration. Nature. 2008 May 15;453(7193):314–21.

Borges GÁ, Elias ST, da Silva SMM, Magalhães PO, Macedo SB, Ribeiro APD, et al. In vitro evaluation of wound healing and antimicrobial potential of ozone therapy. J Cranio-Maxillofacial Surg. 2017;45(3):364–70.

Tanasale ML. Aplikasi Starter Ragi Tape Terhadap Rendemen Dan Mutu Virgin Coconut Oil (VCO). Ekosains. 2012;2(1):47–52.

Fitri R. Formulasi Krim Anti-Aging Yang Mengandung Minyak Kelapa Murni (Virgin Coconut Oil). Universitas Sumatera Utara; 2017.

Cristianti L, Prakosa AH. Pembuatan Minyak Kelapa Murni (Virgin Coconut Oil) menggunakan Fermentasi Ragi Tempe. Universitas Sebelas Maret; 2009.

HARLINANDA SN. OPTIMASI FORMULASI KRIM ANTIOKSIDAN VITAMIN E (DL-ALFA TOKOFEROL ASETAT) DENGAN VCO (VIRGIN COCONUT OIL) (Variasi Kadar VCO 15%, 30%, dan 45% dalam Basis Cold Cream). Universitas Muhammadiyah Malang; 2017.

Nevin KG, Rajamohan T. Effect of Topical Application of Virgin Coconut Oil on Skin Components and Antioxidant Status during Dermal Wound Healing in Young Rats. Skin Pharmacol Physiol. 2010;23(6):290–7.

Soliman AM, Lin TS, Ghafar NA, Das S. Virgin coconut oil and diabetic wound healing: histopathological and biochemical analysis. Eur J Anat. 2018;22(2):135–44.

De Monte A, Gori C. Major ozonated autohaemotherapy in the treatment of limb ulcers not responding to conventional therapy. Vol. 10, International Journal of Ozone Therapy. 2011. 85–98 p.

Degli Agosti I, Ginelli E, Mazzacane B, Peroni G, Bianco S, Guerriero F, et al. Effectiveness of a Short-Term Treatment of Oxygen-Ozone Therapy into Healing in a Posttraumatic Wound. Case Rep Med. 2016;2016:1–5.

Penn JW, Grobbelaar AO, Rolfe KJ. The role of the TGF-? family in wound healing, burns and scarring: a review. Int J Burn Trauma. 2012;2(1):18–28.

Tanggo VTIP. Pengaruh Pemberian Topikal Ekstrak Kulit Delima Pada Penyembuhan Luka Split Thickness Kulit Tikus. Universitas Airlangga; 2013.

Rijal S, Nur M. ANALISA PENGARUH OZONISASI HASIL LUCUTAN PLASMA BERPENGHALANG DIELEKTRIK PADA BERAS TERHADAP PERUBAHAN AMILOGRAFI, KEKERASAN, DAN WARNA. Vol. 4, Youngster Physics Journal. 2015.

Teke S, Nur M. Produksi Ozon Dalam Reaktor Dielectric Barrier Discharge Plasma (DBDP) Terkait Panjang Reaktor Dan Laju Alir Udara. Berk Fis. 2014;17(1):25–32.

Sega A, Zanardi I, Chiasserini L, Gabbrielli A, Bocci V, Travagli V. Properties of sesame oil by detailed 1H and 13C NMR assignments before and after ozonation and their correlation with iodine value, peroxide value, and viscosity measurements. Chem Phys Lipids. 2010 Feb;163(2):148–56.

Valacchi G, Lim Y, Belmonte G, Miracco C, Zanardi I, Bocci V, et al. Ozonated sesame oil enhances cutaneous wound healing in SKH1 mice. Wound Repair Regen. 2011 Jan 1;19(1):107–15.

Kim HS, Noh SU, Han YW, Kim KM, Kang H, Kim HO, et al. Therapeutic effects of topical application of ozone on acute cutaneous wound healing. J Korean Med Sci. 2009 Jun;24(3):368–74.

Zhang J, Guan M, Xie C, Luo X, Zhang Q, Xue Y. Increased growth factors play a role in wound healing promoted by noninvasive oxygen-ozone therapy in diabetic patients with foot ulcers. Oxid Med Cell Longev. 2014;2014.

Published
2021-07-15
Section
Original Article